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Image Search Results
Journal: Springer Protocols Handbooks
Article Title: Animal Models of Acute Neurological Injuries II
doi: 10.1007/978-1-61779-782-8
Figure Lengend Snippet: Fig. 3. APP staining to show the temporal and spatial analysis of axonal damage after focal cerebral ischemia. Accumulation of amyloid precursor protein (APP) was monitored with a rabbit polyclonal anti-APP antibody ( red ) and compared with staining for normal myelin using a mouse monoclonal anti-MBP ( green ). ( a – c ) Representative confocal images of sections double labeled for MBP and APP in the core of the infarct ( left ). The center is out of view to the left. In the merged images from the edge of the infarct ( right ), arrowheads surround a myelinated bundle containing puncta of APP staining. Scale bars = 100 µ m (adapted with permission and modifi cation from ref. 1 ).
Article Snippet: 1° Antibodies—same as for Western blot protocol: (a) 4-HNE: Monoclonal antibody (JaICA, Cat# MHN-020P). (b)
Techniques: Staining, Labeling